Currently, the metabolic syndrome MS is occurring at growing rates worldwide, raising extensive concerns on the mechanisms and therapeutic interventions for this disorder. Herein, we described a novel method of establishing MS model in rodents. Metabolic phenotypes were assessed by glucose tolerance test, insulin tolerance test and hyperinsulinemic-euglycemic clamp. Blood pressure was measured by a tail-cuff system. At the end of the experiment, mice were sacrificed, and blood and tissues were harvested for subsequent analysis. The HFHF diet-fed ICR mice exhibited obvious characteristics of the components of MS, including obvious obesity, severe insulin resistance, hyperinsulinemia, dislipidemia, significant hypertension and hyperuricemia. According to the International Diabetes Federation IDF definition, this syndrome is usually defined as a cluster of metabolic abnormalities, including abdominal obesity, insulin resistance, and dyslipidemia, hypertension, fatty liver disease and type 2 diabetes mellitus [ 10 ].
Basic Res Cardiol. Materials and Methods Reagents Unless otherwise stated, all reagents were purchased from Sigma-aldrich. This method more closely mimics the pathogenesis happened in human MS, and would be an effective and reliable tool for both mechanistic and treatment studies of MS. In contrast, ND rats showed a decrease in insulin sensitivity, associated with glucose intolerance, with increasing age, thus minimizing the effects provided by the HFD. The link between metabolic abnormalities and endothelial dysfunction in type 2 diabetes: an update. Metabolic control was determined by measuring body weight, fasting blood glucose, C-peptide, HOMA2-IR, leptin, and cholesterol; oxidative parameters were studied by lipid peroxidation and total antioxidant capacity in plasma and the use of ROS labelling on tissue. Ibrahim, S.
Table 2 Evolution of high important mice of nonalcoholic fatty liver disease NAFLD, associated with. Lao-Ong, Fructose. In fact, there have emerged multiple types of animal models that commonly used to study obesity, insulin resistance, and oxidative. High semiquantitative determination of the fat expression of certain genes in mouse brain was performed using pairs of primers specific. . diet